Composite

Part:BBa_K4999001

Designed by: Piyush Nanda   Group: iGEM23_Harvard   (2023-10-11)


LuxR driven by constitutive promoter

JUMP compatible LuxR transcriptional unit with constitute promoter. We used J23100 promoter from Anderson's collection, BCD12 Ribosome binding site and B0015 terminator. This part can be assembled with other components of the quorum sensing circuit into a single pJUMP2 plasmid by Golden gate assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal NheI site found at 41
    Illegal NheI site found at 850
    Illegal NheI site found at 873
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 829
    Illegal BsaI.rc site found at 2
    Illegal SapI.rc site found at 97


[edit]
Categories
//awards/composite_part
//cds/transcriptionalregulator/activator
Parameters
None